ERK1/2 Polyclonal antibody

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  WB analysis using 11257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.

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  WB analysis using 11257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.

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  IP experiment of HEK-293 using 11257-1-AP

  IP result of anti-ERK1/2 (IP:11257-1-AP, 4ug; Detection:11257-1-AP 1:3000) with HEK-293 cells lysate 1440 ug.

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  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 11257-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ERK1/2 antibody (11257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 11257-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11257-1-AP

Publications(427)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ERK, ERK1, ERK2, EC:2.7.11.24, ERK-1

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Tested Applications

• Positive WB detected in: PC-12 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse lung tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 419 publications below
• IHC: See 7 publications below
• IF: See 14 publications below
• CoIP: See 2 publications below

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, zebrafish, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERK1/2 fusion protein Ag1759
• Full Name: mitogen-activated protein kinase 3
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38-44 kDa
• GenBank Accession Number: BC013992
• Gene Symbol: ERK1
• Gene ID (NCBI): 5595
• RRID: AB_2139822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer; Authors - Yurou Chen
mouse	WB	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong
human	WB	Redox Biol; Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species; Authors - He Zhao
	WB	Cancer Res; Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells; Authors - Li Xia
mouse	WB	EMBO Mol Med; Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice; Authors - Huiting Wang
mouse	WB	ACS Appl Mater Interfaces; Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels.; Authors - Chaoqun Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (08-15-2024)

The company suggests us this ERK ab as the other one we bought from them was not working. I overlaid ERK with pERK and it matches nicely. Satisfied of this antibody. Done in milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: HT29

ERK1/2 Antibody Western Blot validation (1/5000 dilution) in HT29 (Cat no:11257-1-AP)

FH

XB (Verified Customer) (10-29-2020)

A reliable ab to detect total ERK1/2 by Western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain

ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)