|Positive WB detected in||HEK-293 cells, A375 cells, HepG2 cells, HeLa cells, A549 cells, mouse lung tissue|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A375 cells, MCF-7 cells, PC-3 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11257-1-AP targets ERK1/2 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ERK1/2 fusion protein Ag1759|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||38-44 kDa|
|GenBank accession number||BC013992|
|Gene ID (NCBI)||5595|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
|Product Specific Protocols|
|WB protocol for ERK1/2 antibody 11257-1-AP||Download protocol|
|IHC protocol for ERK1/2 antibody 11257-1-AP||Download protocol|
|IF protocol for ERK1/2 antibody 11257-1-AP||Download protocol|
|FC protocol for ERK1/2 antibody 11257-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
XB (Verified Customer) (10-29-2020)
A reliable ab to detect total ERK1/2 by Western blotting.