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- KD/KO Validated
ERK1/2 Polyclonal antibody
ERK1/2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Rat and More (4)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
183
Cat no : 11257-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells, HeLa cells, HEK-293 cells, PC-12 cells, C6 cells, mouse brain tissue, mouse lung tissue |
Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A375 cells, MCF-7 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 180 publications below |
IHC | See 2 publications below |
IF | See 7 publications below |
Product Information
11257-1-AP targets ERK1/2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, Rat samples.
Tested Reactivity | human, mouse, Rat |
Cited Reactivity | human, mouse, rat, sheep, pig, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ERK1/2 fusion protein Ag1759 |
Full Name | mitogen-activated protein kinase 3 |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 38-44 kDa |
GenBank accession number | BC013992 |
Gene symbol | MAPK3 |
Gene ID (NCBI) | 5595 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
IHC protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
IF protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Appl Mater Interfaces Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels. | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A | ||
Pharmacol Res Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex | ||
Theranostics Precise and efficient silencing of mutant KrasG12D by CRISPR-CasRx controls pancreatic cancer progression. | ||
Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis | ||
J Med Chem Persistent Degradation of HER2 Protein by Hybrid nanoPROTAC for Programmed Cell Death |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH XB (Verified Customer) (10-29-2020) | A reliable ab to detect total ERK1/2 by Western blotting.
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