|Positive WB detected in||HeLa cells, human placenta tissue|
|Positive IHC detected in||human pancreas cancer tissue, human oesophagus tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11261-2-AP targets ERO1LB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ERO1LB fusion protein Ag1776|
|Full Name||ERO1-like beta (S. cerevisiae)|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||40~50 kDa|
|GenBank accession number||BC032823|
|Gene ID (NCBI)||56605|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.
Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease.
Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.
J Biol Chem
Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells.
Hum Mol Genet
Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice.