ERO1LB Polyclonal antibody

• Featured Product
• KD/KO Validated

ERO1LB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11261-2-AP

Synonyms

ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta

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Validation Data Gallery

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  WB analysis of HeLa using 11261-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11261-2-AP (ERO1LB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11261-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11261-2-AP (ERO1LB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11261-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11261-2-AP (ERO1LB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 11261-2-AP (ERO1LB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 11261-2-AP (ERO1LB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human oesophagus using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 11261-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11261-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11261-2-AP (ERO1LB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue
• Positive IHC detected in: human pancreas cancer tissue, human oesophagus tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below

Product Information

11261-2-AP targets ERO1LB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERO1LB fusion protein Ag1776
• Full Name: ERO1-like beta (S. cerevisiae)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 40~50 kDa
• GenBank Accession Number: BC032823
• Gene Symbol: ERO1LB
• Gene ID (NCBI): 56605
• RRID: AB_2101503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Curcumin Facilitates a Transitory Cellular Stress Response in Trembler-J Mice.; Authors - Okamoto Yuji Y
mouse	WB	Endocrinology; PMID: 21540283; Authors - Khoo Cynthia C ; KD Validated
human	WB	J Biol Chem; Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells.; Authors - Rajpal Gautam G
human	WB	Sci Rep; Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells.; Authors - Norio Takei ; KO Validated
mouse	WB	J Toxicol; Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease.; Authors - Galligan James J JJ
human	WB	ACS Bio Med Chem Au; Identification of Natural Product Sulfuretin Derivatives as Inhibitors for the Endoplasmic Reticulum Redox Protein ERO1α; Authors - Brennan D Johnson ; KO Validated