ERO1LB Polyclonal antibody

ERO1LB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18312-1-AP

Synonyms

ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta

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Validation Data Gallery

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  WB analysis of human brain using 18312-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 18312-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 18312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 18312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18312-1-AP targets ERO1LB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ERO1-like beta (S. cerevisiae)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC032823
• Gene Symbol: ERO1LB
• Gene ID (NCBI): 56605
• RRID: AB_2101502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERO1LB is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. It is required for the folding of cell, thereby being a source of oxidative stress. This antibody has no cross reaction to ERO1L.