ERO1LB Recombinant monoclonal antibody

• 

  WB analysis using 86104-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86104-1-RR (ERO1LB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ERO1LB Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86104-1-RR

Clone No.250655G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:1.8.4.-, Endoplasmic reticulum oxidoreductase beta, Endoplasmic reticulum oxidoreductin-1-like protein B, ERO1B, Ero1beta

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Tested Applications

• Positive WB detected in: PC-3 cells, IM-9 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:6000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86104-1-RR targets ERO1LB in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2622

  Product name: recombinant human ERO1LB protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 34-467 aa of NM_019891.3

  Sequence: QVTGVLDDCLCDIDSIDNFNTYKIFPKIKKLQERDYFRYYKVNLKRPCPFWAEDGHCSIKDCHVEPCPESKIPVGIKAGHSNKYLKMANNTKELEDCEQANKLGAINSTLSNQSKEAFIDWARYDDSRDHFCELDDERSPAAQYVDLLLNPERYTGYKGTSAWRVWNSIYEENCFKPRSVYRPLNPLAPSRGEDDGESFYTWLEGLCLEKRVFYKLISGLHASINLHLCANYLLEETWGKPSWGPNIKEFKHRFDPVETKGEGPRRLKNLYFLYLIELRALSKVAPYFERSIVDLYTGNAEEDADTKTLLLNIFQDTKSFPMHFDEKSMFAGDKKGAKSLKEEFRLHFKNISRIMDCVGCDKCRLWGKLQTQGLGTALKILFSEKEIQKLPENSPSKGFQLTRQEIVALLNAFGRLSTSIRDLQNFKVLLQHSR

• Full Name: ERO1-like beta (S. cerevisiae)
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: NM_019891.3
• Gene Symbol: ERO1LB
• Gene ID (NCBI): 56605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86YB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoplasmic reticulum oxidoreductases (Eros) are essential for the formation of disulfide bonds. Mammals express two related Ero proteins, Ero1L alpha and Ero1L beta. The two homologous proteins can form homodimers and mixed heterodimers. ERO1-Lα and -β are highly expressed in organs with a high demand for secretion, consistent with their roles in oxidative folding of ER proteins.