ESAM Polyclonal antibody

ESAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12445-1-AP

Synonyms

ESAM, W117m

---

Validation Data Gallery

• 

  WB analysis of human lung using 12445-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12445-1-AP (ESAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12445-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12445-1-AP (ESAM antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: human lung tissue, human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12445-1-AP targets ESAM in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESAM fusion protein Ag2962
• Full Name: endothelial cell adhesion molecule
• Calculated Molecular Weight: 390 aa, 41 kDa
• Observed Molecular Weight: 56-58 kDa
• GenBank Accession Number: BC016868
• Gene Symbol: ESAM
• Gene ID (NCBI): 90952
• RRID: AB_2101666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Oncolytics; miR-7 Reduces Breast Cancer Stem Cell Metastasis via Inhibiting RELA to Decrease ESAM Expression.; Authors - Miao Li