ESF1 Polyclonal antibody

ESF1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23496-1-AP

Synonyms

ABT1 associated protein, ABTAP, C20orf6, ESF1, ESF1 homolog, HDCMC28P

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 23496-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23496-1-AP (ESF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 23496-1-AP

  Immunofluorescent analysis of MCF-7 cells using 23496-1-AP (ESF1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

---

Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23496-1-AP is ESF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESF1 fusion protein Ag19037
• Full Name: ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae)
• Calculated Molecular Weight: 851 aa, 99 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC146542
• Gene Symbol: ESF1
• Gene ID (NCBI): 51575
• RRID: AB_2879287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Clin Proteomics; ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis; Authors - Qing Yu
human	IF	bioRxiv; Mapping and engineering RNA-controlled architecture of the multiphase nucleolus; Authors - S A Quinodoz