Tested Applications
| Positive WB detected in | COLO 320 cells, mouse lung tissue, MCF-7 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human lung tissue, mouse colon tissue, human bowen disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21045-1-AP targets ESRP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15386 Product name: Recombinant human ESRP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-226 aa of BC099916 Sequence: EKELILLFWKVVDLANKKVGQLHEVLVRPDQLELTEDCKEETKIDVESLSSASQLDQALRQFNQSVSNELNIGVGTSFCLCTDGQLHVRQILHPEASKKNVLLPECFYSFFDLRKEFKKCCPGSPDIDKLDVATMTEYLNFEKSSSVSRYGASQVEDMGNIILAMISEPYNHRFSDPERVNYKFESGTCSKMELIDDNTV Predict reactive species |
| Full Name | epithelial splicing regulatory protein 1 |
| Calculated Molecular Weight | 681 aa, 76 kDa |
| Observed Molecular Weight | 76 kDa |
| GenBank Accession Number | BC099916 |
| Gene Symbol | ESRP1 |
| Gene ID (NCBI) | 54845 |
| RRID | AB_2878801 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6NXG1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ESRP1, also named as Epithelial splicing regulatory protein 1, is a 681 amino acid protein, which belongs to the ESRP family. ESRP1 as a mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. ESRP1 specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). ESRP1 exists various isoforms and molecular weight of each isoform is 76 kDa, 67 kDa, and 73 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| IHC protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| IP protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| WB protocol for ESRP1 antibody 21045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ESRP1 Polyclonal antibody (Cat# 21045-1-AP) has 13 citations published in journals including Cancer Research, Nucleic Acids Research, Cell Death & Differentiation, Molecular Therapy, Oncogene, Cell Death Discovery, Journal of Experimental & Clinical Cancer Research, Cell Signaling, eLife, Science Advances, Frontiers in Medicine, and American Journal of Cancer Research. Research fields span alternative splicing, ferroptosis, epithelial-mesenchymal transition, circRNA biology, and multiple cancer types (lung, gastric, colorectal, ovarian, breast, prostate, and pancreatic).
| Species | Application | Title |
|---|---|---|
Cancer Res MEN1 Promotes Ferroptosis by Disrupting CD44 Alternative Splicing to Suppress Lung Cancer | ||
Nucleic Acids Res ESRP1 controls biogenesis and function of a large abundant multiexon circRNA
| ||
J Exp Clin Cancer Res ESRP1 exerts anti-tumor role by promoting ferroptosis in diffuse-type gastric cancer | ||
Cell Death Differ Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop. | ||
Mol Ther circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis.
| ||
Cell Death Discov Circ-NOLC1 promotes epithelial ovarian cancer tumorigenesis and progression by binding ESRP1 and modulating CDK1 and RhoA expression.
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