ESX1 Polyclonal antibody

ESX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14657-1-AP

Synonyms

ESX homeobox 1, ESX1, ESX1L, ESX1R, ESXR1, Homeobox protein ESX1

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Validation Data Gallery

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  WB analysis of mouse brain using 14657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14657-1-AP (ESX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14657-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14657-1-AP (ESX1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14657-1-AP

  IP result of anti-ESX1 (IP:14657-1-AP, 4ug; Detection:14657-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14657-1-AP targets ESX1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESX1 fusion protein Ag6269
• Full Name: ESX homeobox 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC053599
• Gene Symbol: ESX1
• Gene ID (NCBI): 80712
• RRID: AB_10694138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESX1, also named as ESX1L and ESX1R , is an X-linked homeobox gene primarily expressed in the placenta and testis, with physiological functions in placenta/fetus development and spermatogenesis. It has a substantively different C-terminal region subject to strong purifying selection. ESX1 can be cleaved into 2 part: ESXR1-N(45kd) and ESXR1-C(20kd). ESXR1-N acts as a transcriptional repressor. ESXR1-C has the ability to inhibit cyclin turnover.