Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293T cells, HSC-T6 cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67688-1-Ig targets ESYT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16840 Product name: Recombinant human FAM62A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 755-1104 aa of BC004998 Sequence: DVPSGRLHLRLERLTPRPTAAELEEVLQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTKHLSPYATLTVGDSSHKTKTISQTSAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSLSLPLSELLVADQLCLDRWFTLSSGQGQVLLRAQLGILVSQHSGVEAHSHSYSHSSSSLSEEPELSGGPPHITSSAPELRQRLTHVDSPLEAPAGPLGQVKLTLWYYSEERKLVSIVHGCRSLRQNGRDPPDPYVSLLLLPDKNRGTKRRTSQKKRTLSPEFNERFEWELPLDEAQRRKLDVSVKSNSSFMSRERELLGKVQLDLAETDLSQGVARWYDLMDNKDKGSS Predict reactive species |
| Full Name | family with sequence similarity 62 (C2 domain containing), member A |
| Calculated Molecular Weight | 1104 aa, 123 kDa |
| Observed Molecular Weight | 123 kDa |
| GenBank Accession Number | BC004998 |
| Gene Symbol | ESYT1 |
| Gene ID (NCBI) | 23344 |
| RRID | AB_2882881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BSJ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ESYT1 antibody 67688-1-Ig | Download protocol |
| WB protocol for ESYT1 antibody 67688-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ESYT1 Monoclonal antibody (2C12E4), catalog number 67688-1-Ig, has 2 total citations. These appear in Nature Cell Biology (2025) and Frontiers in Cell and Developmental Biology (2022). The cited research spans sphingosine-1-phosphate signaling and HDL-derived cholesterol transport, as well as plasma membrane wound repair involving S100A11–annexin interactions. Both studies used the antibody for Western blotting, with one also employing immunofluorescence, in human samples.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Sphingosine-1-phosphate signalling activates E-Syt1 to facilitate HDL-derived cholesterol transport
| ||
Front Cell Dev Biol The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair
|
Reviews
What customers say
The single review rates this antibody 4 out of 5 stars for Western Blot. Used at a 1:500 dilution to detect recombinant Esyt1 in HEK293 lysate, it produced a clean, correctly sized band on the first attempt. The reviewer expressed satisfaction with the specificity and signal quality, and plans to optimize the dilution further while exploring additional applications and primary cell lysates.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shihab (Verified Customer) (09-17-2021) | This antibody was used in a western blot application to identify recombinant Esyt1 prescence. Using 1:500 at the first attempt, this antibody was able to successfully provide a band at the correct size. Furthermore, the band was clean which was an added bonus. I will be reducing the concentration of antibody used and also trying this antibody in other applications as well as primary cell lysate
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