ETFA Monoclonal antibody

ETFA Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3C12G5

Cat no : 67661-1-Ig

Synonyms

Alpha ETF, EMA, ETFA, GA2, MADD

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Validation Data Gallery

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  WB analysis using 67661-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67661-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 67661-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

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Tested Applications

• Positive WB detected in: LNCaP cells, mouse brain tissue, HuH-7 cells, HeLa cells, K-562 cells, pig liver tissue, rat liver tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Product Information

67661-1-Ig targets ETFA in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ETFA fusion protein Ag30257
• Full Name: electron-transfer-flavoprotein, alpha polypeptide
• Calculated molecular weight: 30 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC015526
• Gene symbol: ETFA
• Gene ID (NCBI): 2108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)