ETFA Monoclonal antibody
ETFA Monoclonal Antibody for IF, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
WB, IF,ELISA
Conjugate
Unconjugated
CloneNo.
3C12G5
Cat no : 67661-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, mouse brain tissue, HuH-7 cells, HeLa cells, K-562 cells, pig liver tissue, rat liver tissue |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
67661-1-Ig targets ETFA in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
Tested Reactivity | Human, mouse, rat, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | ETFA fusion protein Ag30257 |
Full Name | electron-transfer-flavoprotein, alpha polypeptide |
Calculated molecular weight | 30 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC015526 |
Gene symbol | ETFA |
Gene ID (NCBI) | 2108 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)
Protocols
Product Specific Protocols | |
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WB protocol for ETFA antibody 67661-1-Ig | Download protocol |
IF protocol for ETFA antibody 67661-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |