ETFA Monoclonal antibody

ETFA Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3C12G5

Cat no : 67661-1-Ig

Synonyms

Alpha ETF, EMA, ETFA, GA2, MADD



Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue, HuH-7 cells, HeLa cells, K-562 cells, pig liver tissue, rat liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67661-1-Ig targets ETFA in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ETFA fusion protein Ag30257
Full Name electron-transfer-flavoprotein, alpha polypeptide
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC015526
Gene symbol ETFA
Gene ID (NCBI) 2108
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)

Protocols

Product Specific Protocols
WB protocol for ETFA antibody 67661-1-IgDownload protocol
IF protocol for ETFA antibody 67661-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols