CoraLite® Plus 594-conjugated ETFA Monoclonal antibody

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  IF Staining of HeLa using CL594-67661

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 594 ETFA antibody (CL594-67661, Clone: 3C12G5 ) at dilution of 1:400.

ETFA Monoclonal Antibody for IF/ICC

Cat No. CL594-67661

Clone No.3C12G5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

3C12G5, Alpha ETF, Alpha-ETF, EMA, GA2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67661 targets ETFA in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30257

  Product name: Recombinant human ETFA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-333 aa of BC015526

  Sequence: MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

• Full Name: electron-transfer-flavoprotein, alpha polypeptide
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015526
• Gene Symbol: ETFA
• Gene ID (NCBI): 2108
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13804
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)