ETHE1 Polyclonal antibody

ETHE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27786-1-AP

Synonyms

ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12

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Validation Data Gallery

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  WB analysis of BGC-823 using 27786-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27786-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27786-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 27786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 27786-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: BGC-823 cells, HeLa cells, mouse liver tissue
• Positive IHC detected in: human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

27786-1-AP targets ETHE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETHE1 fusion protein Ag26597
• Full Name: ethylmalonic encephalopathy 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC008250
• Gene Symbol: ETHE1
• Gene ID (NCBI): 23474
• RRID: AB_2880971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.

Publications

Species	Application	Title
human	WB,IHC	Cell Death Dis; Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma; Authors - Yiwei Liu