EVL Polyclonal antibody

EVL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13484-1-AP

Synonyms

Ena/VASP like protein, Enah/Vasp like, EVL, RNB6

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 13484-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13484-1-AP (EVL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 13484-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 13484-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13484-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13484-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human small intestine tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13484-1-AP targets EVL in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EVL fusion protein Ag4302
• Full Name: Enah/Vasp-like
• Calculated Molecular Weight: 418 aa, 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC032358
• Gene Symbol: EVL
• Gene ID (NCBI): 51466
• RRID: AB_10644121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997).

Publications

Species	Application	Title
human	WB	J Pers Med; Immunocyte Infiltration Analysis and Immunohistochemistry Identify EVL as a Potential Prognostic Biomarker for Pancreatic Cancer; Authors - Yan Du
mouse	IHC	Clin Transl Med; Genetic and pharmacological inhibition of METTL3 alleviates renal fibrosis by reducing EVL m6A modification through an IGF2BP2-dependent mechanism; Authors - Wei-Jian Ni