EXD1 Polyclonal antibody

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  WB analysis of HeLa using 17313-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17313-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17313-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17313-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 17313-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXD1 antibody (17313-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

EXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17313-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

piRNA biogenesis protein EXD1, Inactive exonuclease EXD1, Exonuclease 3'-5' domain-like-containing protein 1, Exonuclease 3'-5' domain-containing protein 1, EXDL1

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17313-1-AP targets EXD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11282

  Product name: Recombinant human EXD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 164-514 aa of BC030628

  Sequence: SDCLSHQYGILLNNVFDTQVADVLQFSMETGGYLPNCITTLQESLIKHLQVAPKYLSFLEKRQKLIQENPEVWFIRPVSPSLLKILALEATYLLPLRLALLDEMMSDLTTLVDGYLNTYREGSADRLGGTEPTCMELPEELLQLKDFQKQRREKAAREYRVNAQGLLIRTVLQPKKLVTETAGKEEKVKGFLFGKNFRIDKAPSFTSQDFHGDVNLLKEESLNKQATNPQHLPPTEEGETSEDSSNKLICTKSKGSEDQRITQKEHFMTPKHEFQASLSLKEETEQLLMVENKEDLKCTKQAVSMSSFPQETRVSPSDTFYPIRKTVVSTLPPCPALEKIDSWISPFLNLP

• Full Name: exonuclease 3'-5' domain containing 1
• Calculated Molecular Weight: 514 aa, 58 kDa
• Observed Molecular Weight: 36 kDa, 58 kDa, 75 kDa
• GenBank Accession Number: BC030628
• Gene Symbol: EXD1
• Gene ID (NCBI): 161829
• RRID: AB_2878379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NHP7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXD1, also named as EXDL1, has three isoforms with MW 58 kDa, 36 kDa and 65 kDa. This antibody can recognize all these three isoforms.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Andrology; Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes; Authors - Opeyemi Olotu