EXD1 Polyclonal antibody

EXD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17313-1-AP

Synonyms

piRNA biogenesis protein EXD1, Inactive exonuclease EXD1, Exonuclease 3'-5' domain-like-containing protein 1, Exonuclease 3'-5' domain-containing protein 1, EXDL1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 17313-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17313-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17313-1-AP (EXD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17313-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17313-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17313-1-AP (EXD1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 17313-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EXD1 antibody (17313-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17313-1-AP targets EXD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXD1 fusion protein Ag11282
• Full Name: exonuclease 3'-5' domain containing 1
• Calculated Molecular Weight: 514 aa, 58 kDa
• Observed Molecular Weight: 36 kDa, 58 kDa, 75 kDa
• GenBank Accession Number: BC030628
• Gene Symbol: EXD1
• Gene ID (NCBI): 161829
• RRID: AB_2878379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXD1, also named as EXDL1, has three isoforms with MW 58 kDa, 36 kDa and 65 kDa. This antibody can recognize all these three isoforms.

Publications

Species	Application	Title
mouse	WB,IF	Andrology; Intermitochondrial cement (IMC) harbors piRNA biogenesis machinery and exonuclease domain-containing proteins EXD1 and EXD2 in mouse spermatocytes; Authors - Opeyemi Olotu