EXOSC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXOSC3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (2)

Cat no : 15062-1-AP

Synonyms

Ribosomal RNA-processing protein 40, Exosome complex component RRP40, EXOSC 3, CGI-102

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Validation Data Gallery

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  WB analysis using 15062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 15062-1-AP

  IP result of anti-EXOSC3 (IP:15062-1-AP, 4ug; Detection:15062-1-AP 1:500) with A2780 cells lysate 800ug.

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  IHC staining of human colon cancer using 15062-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15062-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15062-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using EXOSC3 antibody (15062-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A2780 cells, HEK-293T cells, PC-3 cells, NIH/3T3 cells, mouse spleen tissue
• Positive IP detected in: A2780 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 2 publications below

Product Information

15062-1-AP targets EXOSC3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC3 fusion protein Ag7065
• Full Name: exosome component 3
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC002437
• Gene Symbol: EXOSC3
• Gene ID (NCBI): 51010
• RRID: AB_2278183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].

Publications

Species	Application	Title
human		Mol Cell; Integrator is a genome-wide attenuator of non-productive transcription.; Authors - Søren Lykke-Andersen ; KD Validated
mouse	WB	Nucleic Acids Res; The RNA exosome contributes to gene expression regulation during stem cell differentiation.; Authors - Marta Lloret-Llinares
human	WB	Nucleic Acids Res; The RNA exosome shapes the expression of key protein-coding genes.; Authors - Mengjun Wu
human	WB	Nucleic Acids Res; A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.; Authors - Katarzyna Kalisiak
human	WB	Nat Commun; EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.; Authors - Veronika Boczonadi
human	WB	Nucleic Acids Res; A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.; Authors - Katarzyna Kalisiak

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (06-01-2022)

Comparison of WT and Exosc3 depletion showed a reduction in Exosc3 protein levels (confirming the specificity of the antibodies). Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC3 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:15062-1-AP)

FH

Nikolaus (Verified Customer) (05-24-2022)

Exosc3 conditional KO timecourse; Hsp70 as loading control; exoscenllent abs

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: mESC

EXOSC3 Antibody Western Blot validation (1/5000 dilution) in mESC (Cat no:15062-1-AP)