Tested Applications
| Positive WB detected in | HEK-293 cells, Raji cells, HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:350-1:1400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
Product Information
15627-1-AP targets EXOSC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8098 Product name: Recombinant human EXOSC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC007742 Sequence: MEEEMHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS Predict reactive species |
| Full Name | exosome component 5 |
| Calculated Molecular Weight | 235 aa, 25 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC007742 |
| Gene Symbol | EXOSC5 |
| Gene ID (NCBI) | 56915 |
| RRID | AB_2293809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQT4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CML28 is a component of the human exosome, which is a multiprotein complex involved in the 3′ processing and degradation of RNA [PMID:12359762]. CML28 is a novel 28 kDa TAA identified by serological analysis of cDNA expression libraries (SEREX) screening of a chronic myeloid leukemia (CML) cDNA expression library. As a potential target for tumor-specific immunotherapy, CML28 is superior to other known TAAs characterized by its broader spectrum expression in tumor cells and stricter expression in normal tissues [PMID:16815301].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| IHC protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| IP protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| WB protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cancer EXOSC5 promotes proliferation of gastric cancer through regulating AKT/STAT3 signaling pathways. | ||
Int J Mol Sci Integrated Bioinformatic Investigation of EXOSCs in Hepatocellular Carcinoma Followed by the Preliminary Validation of EXOSC5 in Cell Proliferation | ||
Life Sci Alliance Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tsimafei (Verified Customer) (07-06-2022) | Reduction of Exosc5 is observed when comparing depletion vs control. Antibodies were incubated ON at 4C.
 |
FH Nikolaus (Verified Customer) (05-24-2022) | Depletion is a conditional KO time-course; strong cross-reactivity with Bio-Rad dual stain ladder (25 kDa band)
 |
