Tested Applications
| Positive WB detected in | HEK-293 cells, Raji cells, HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:350-1:1400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15627-1-AP targets EXOSC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8098 Product name: Recombinant human EXOSC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC007742 Sequence: MEEEMHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS Predict reactive species |
| Full Name | exosome component 5 |
| Calculated Molecular Weight | 235 aa, 25 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC007742 |
| Gene Symbol | EXOSC5 |
| Gene ID (NCBI) | 56915 |
| RRID | AB_2293809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQT4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CML28 is a component of the human exosome, which is a multiprotein complex involved in the 3′ processing and degradation of RNA [PMID:12359762]. CML28 is a novel 28 kDa TAA identified by serological analysis of cDNA expression libraries (SEREX) screening of a chronic myeloid leukemia (CML) cDNA expression library. As a potential target for tumor-specific immunotherapy, CML28 is superior to other known TAAs characterized by its broader spectrum expression in tumor cells and stricter expression in normal tissues [PMID:16815301].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| IHC protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| IP protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| WB protocol for EXOSC5 antibody 15627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EXOSC5 Polyclonal antibody (Cat# 15627-1-AP) has 8 total citations. These appear in journals including Molecular Cell, EMBO Journal, International Journal of Molecular Science, BMC Cancer, Journal of Cancer, Life Science Alliance, and Gene. Research fields span RNA exosome biology, hepatocellular carcinoma, clear cell renal cell carcinoma, lung adenocarcinoma, gastric cancer, SARS-CoV-2 infection, and ferroptosis in ovarian cancer. Cited applications include Western blot and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci H3K4me3 and H3K27ac Promote ccRCC Proliferation Through the CDC6-EXOSC5 Axis. | ||
Int J Mol Sci Integrated Bioinformatic Investigation of EXOSCs in Hepatocellular Carcinoma Followed by the Preliminary Validation of EXOSC5 in Cell Proliferation | ||
J Cancer EXOSC5 promotes proliferation of gastric cancer through regulating AKT/STAT3 signaling pathways. |
Reviews
What customers say
Both reviewers rated the antibody 5 stars for Western Blot on mESCs, using dilutions of 1:5000 and 1:2000. Each confirmed a clear reduction of Exosc5 signal in depletion versus control samples, with overnight incubation at 4 °C. One reviewer noted strong cross-reactivity with the Bio-Rad dual stain ladder at the 25 kDa band, which should be considered when interpreting results. Overall, the antibody performed reliably for detecting Exosc5 knockdown in mouse embryonic stem cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (07-06-2022) | Reduction of Exosc5 is observed when comparing depletion vs control. Antibodies were incubated ON at 4C.
 |
Nikolaus (Verified Customer) (05-24-2022) | Depletion is a conditional KO time-course; strong cross-reactivity with Bio-Rad dual stain ladder (25 kDa band)
 |
