EXTL3 Polyclonal antibody

EXTL3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10588-1-AP

Synonyms

botv, EXTL3, KIAA0519, REG, REGR, RPR

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Validation Data Gallery

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  WB analysis of HepG2 using 10588-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10588-1-AP (EXTL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10588-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10588-1-AP (EXTL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10588-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10588-1-AP (EXTL3 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HepG2 using 10588-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10588-1-AP (EXTL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

10588-1-AP targets EXTL3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXTL3 fusion protein Ag0936
• Full Name: exostoses (multiple)-like 3
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC006363
• Gene Symbol: EXTL3
• Gene ID (NCBI): 2137
• RRID: AB_2262577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Exostosin like glycosyltransferase 3 (EXTL3), also known as RPR, is located at chromosome 8p21.1 and composed of 919 amino acids, making it the longest member of the EXT family (PMID: 35676258). EXTL3 encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS) (PMID: 24128412). EXTL3 has been identified as a receptor molecule for regenerating islet-derived (REG) protein ligands, which have been shown to stimulate islet β-cell growth. Mutations in EXTL3 could lead to skeletal dysplasia, developmental delay, and severe immune deficiency (PMID: 28148688). The predicted molecular weight is 105 kDa, but we detected the band at 65 kDa perhaps due to alternative splicing (PMID: 14618397).

Publications

Species	Application	Title
human	WB,IF	Endocr Pract; Discovery of a human peptide sequence signaling islet neogenesis.; Authors - Levetan Claresa S CS
human,mouse	WB,IF	Ann N Y Acad Sci; REG1A protects retinal photoreceptors from blue light damage; Authors - Ze-Hua Xu