Tested Applications
| Positive WB detected in | HepG2 cells, mouse colon tissue, HuH-7 cells, NCCIT cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22658-1-AP targets EYA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18489 Product name: Recombinant human EYA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC121799 Sequence: MEMQDLTSPHSRLSGSSESPSGPKLGNSHINSNSMTPNGTEVKTEPMSSSETASTTADGSLNNFSGSAIGSSSFSPRPTHQFSPPQIYPSNRPYPHILPTPSSQTMAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYGALWAGIKTEGGLSQSQSPGQTGFLSYGTSFSTPQPGQAPYSYQMQGSSFTTSSGIYTGNNSLTNSSGFNSSQQDYPSYPSFGQGQYAQYYNSSPYPAHYMTSSNTSPTTPSTNATYQLQEPPSGITSQAVTDPTAEYSTIHSPSTPIKDSDSDRLRRGSD Predict reactive species |
| Full Name | eyes absent homolog 1 (Drosophila) |
| Calculated Molecular Weight | 592 aa, 65 kDa |
| Observed Molecular Weight | 61 kDa and 65 kDa |
| GenBank Accession Number | BC121799 |
| Gene Symbol | EYA1 |
| Gene ID (NCBI) | 2138 |
| RRID | AB_2879145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99502 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EYA1 antibody 22658-1-AP | Download protocol |
| IHC protocol for EYA1 antibody 22658-1-AP | Download protocol |
| IP protocol for EYA1 antibody 22658-1-AP | Download protocol |
| WB protocol for EYA1 antibody 22658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 22658-1-AP (anti-EYA1) has 20 citations published in journals including Nature Communications, Cell Reports, International Journal of Biological Sciences, Stem Cell Research & Therapy, Oncotarget, iScience, and others. Associated research fields span oncology (melanoma, glioma, thyroid carcinoma, hepatocellular carcinoma, breast cancer, AML), developmental biology (craniofacial, inner ear, parathyroid differentiation), neuroscience, stem cell biology, and kidney disease.
| Species | Application | Title |
|---|---|---|
Nat Commun The Eyes Absent family members EYA4 and EYA1 promote PLK1 activation and successful mitosis through tyrosine dephosphorylation | ||
Int J Biol Sci Protein phosphatase EYA1 regulates the dephosphorylation and turnover of BCL2L12 to promote glioma development
| ||
Stem Cell Res Ther Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model. | ||
Cell Rep Developmental reprogramming underlies chemotherapy resistance in favorable-histology Wilms tumor. | ||
Cell Rep Retinal determination network reactivation drives chemoresistance and blocks myeloid differentiation in acute myeloid leukemia. | ||
Cell Rep YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis |
