CoraLite® Plus 405 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL405-98013

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (CL405-98013, Clone:230047E5), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL405-98013

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human Endoglin/CD105 Rabbit Recombinant Antibody (CL405-98013, Clone:230047E5)(red), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Endoglin/CD105 Recombinant Antibody for FC

Cat No. CL405-98013

Clone No.230047E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD105, 230047E5, HHT1, ENG, Endoglin

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL405-98013 targets Endoglin/CD105 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: endoglin
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC014271
• Gene Symbol: CD105
• Gene ID (NCBI): 2022
• RRID: AB_3672408
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17813
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)