Endothelin 3 Polyclonal antibody
Endothelin 3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27871-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
Positive IHC detected in | human thyroid cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
27871-1-AP targets Endothelin 3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Endothelin 3 fusion protein Ag27393 |
Full Name | endothelin 3 |
Observed Molecular Weight | 28 kDa |
GenBank Accession Number | BC053866 |
Gene Symbol | Endothelin 3 |
Gene ID (NCBI) | 1908 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Endothelin 3 (EDN3) is also named as ET-3 and Preproendothelin-3 (PPET3). Endothelin 3 belongs to the endothelin/sarafotoxin family. It reported downregulation of EDN3 as a result of methylation of its promoter in breast cancer, and EDN3 level was also decreased in cervical cancer (PMID: 36542159). ET-3 is the most abundant ET peptide in the rat brain, mainly localized to neurons and glia of the neostriatum, hypothalamic nuclei, hippocampus, and Purkinje cells of the cerebellum and medulla oblongata (PMID: 32589590).
Protocols
Product Specific Protocols | |
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WB protocol for Endothelin 3 antibody 27871-1-AP | Download protocol |
IHC protocol for Endothelin 3 antibody 27871-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |