Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88021-1-RR targets Epha4 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg8776 Product name: Recombinant mouse Epha4 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-547 aa of NM_007936.3 Sequence: VTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEASQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRESQFGKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEEQNGECQACKIGYYKALSTDASCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTRVSITDLLAHTNYTFEIWAVNGVSKYNPSPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST Predict reactive species |
| Full Name | Eph receptor A4 |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | NM_007936.3 |
| Gene Symbol | Epha4 |
| Gene ID (NCBI) | 13838 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q03137-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EPH receptor A4 (EPHA4) is a member of the Eph receptor family of receptor tyrosine kinases (RTKs) that interacts with both ephrin-A and ephrin-B ligands, making it one of the most versatile Eph receptors. In mice, Epha4 is highly expressed in the central nervous system, particularly during development, where it regulates axon guidance, neuronal pathfinding, synaptic plasticity, and neural circuit formation. EPHA4 mediates contact-dependent bidirectional signaling, often inducing cell repulsion and boundary formation, which is critical for proper tissue patterning. Beyond development, EPHA4 continues to function in the adult CNS, contributing to synaptic remodeling and neuronal regeneration responses.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Epha4 antibody 88021-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
