Ephrin B2 Polyclonal antibody

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• Ephrin B2 Polyclonal antibody (26533-1-AP)

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  WB analysis using 26533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26533-1-AP (Ephrin B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 26533-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Ephrin B2 antibody (26533-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Ephrin B2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26533-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

EFNB2, EPLG5, EPH-related receptor tyrosine kinase ligand 5, Ephrin-B2, Htk L

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse placenta tissue, mouse spleen tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

26533-1-AP targets Ephrin B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24865

  Product name: Recombinant human EFNB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 168-235 aa of BC074856

  Sequence: ASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGC

• Full Name: ephrin-B2
• Calculated Molecular Weight: 333 aa, 37 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC074856
• Gene Symbol: EFNB2
• Gene ID (NCBI): 1948
• RRID: AB_3085878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52799
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncol Rep; Exploring the role of disulfidptosis‑related signatures in immune microenvironment, prognosis and therapeutic strategies of cholangiocarcinoma.; Authors - Yue Chen ; KD Validated
mouse	WB	Sci Adv; EphB1-NR2B receptor signaling in glutamatergic neurons of the ventroposteromedial thalamic nucleus regulates emergence from anesthesia.; Authors - Yuexin Liu
hamster	WB,IF	Viruses; Ephrin B1 and B2 Mediate Cedar Virus Entry into Egyptian Fruit Bat Cells; Authors - Lea Lenhard ; KD Validated
human,mouse	WB	Cell Signal; siRASA1 in vascular endothelial cells alleviates subretinal fibrosis through inhibiting macrophage-myofibroblast transdifferentiation via SHH signaling pathway.; Authors - Lei Jiang