Factor XI Polyclonal antibody

Factor XI Polyclonal Antibody for WB, ELISA

Cat No. 24321-1-AP

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

F11, FXI, Coagulation factor XIa heavy chain, Coagulation factor XIa light chain, EC:3.4.21.27

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWB

WB analysis using 24321-1-AP
Rat liver tissue were subjected to SDS PAGE followed by western blot with 24321-1-AP (Factor XI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 24321-1-AP
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24321-1-AP (Factor XI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Factor XI in ?

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

rat liver tissue, mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24321-1-AP targets Factor XI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18004 Product name: Recombinant human F11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 58-397 aa of BC119014 Sequence: LFTFTAESPSEDPTRWFTCVLKDSVTETLPRVNRTAAISGYSFKQCSHQISACNKDIYVDLDMKGINYNSSVAKSAQECQERCTDDVHCHFFTYATRQFPSLEHRNICLLKHTQTGTPTRITKLDKVVSGFSLKSCALSNLACIRDIFPNTVFADSNIDSVMAPDAFVCGRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPSTRIKKSKALSGFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAAKSHEACQKLCTNAVRCQFFTYTPAQASCNEGKGKCYLKLSSNGSPTKILHGRGGISGYTLRLCKMDNECTTKIKPRIVGGTASVRG Predict reactive species
Full Name	coagulation factor XI
Calculated Molecular Weight	625 aa, 70 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC119014
Gene Symbol	F11
Gene ID (NCBI)	2160
RRID	AB_3742107
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P03951
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XI (FXI) is the zymogen of a blood coagulation protease, factor XIa (FXIa), that contributes to hemostasis by activating factor IX. Factor XI is a precursor of an S1 serine protease found in blood plasma. When activated to FXIa, it plays a crucial role in forming and stabilizing fibrin by triggering the activation of factor IX (PMID: 38545505). FXI has 2 isoforms with the molecular mass of 70 kDa and 64 kDa. Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.