Mouse F4/80 Recombinant antibody, PBS Only

F4/80 Recombinant Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2P18

Cat no : 81668-1-PBS

Synonyms

DD7A5 7, EGF TM7, Emr1, F4 80, F4/80, Gpf480, Ly71, TM7LN3

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Validation Data Gallery

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  IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 81668-1-RR (same clone as 81668-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 81668-1-RR (same clone as 81668-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81668-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81668-1-PBS targets F4/80 in IF, IHC, Indirect ELISA applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: F4/80 fusion protein Ag28252
• Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_010130
• Gene Symbol: Emr1
• Gene ID (NCBI): 13733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.