F4/80 Recombinant antibody
F4/80 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2P18
Cat no : 81668-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue, RAW 264.7 cells |
Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:4000-1:10000 |
Immunofluorescence (IF)-P | IF-P : 1:750-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 2 publications below |
Product Information
81668-1-RR targets F4/80 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | F4/80 fusion protein Ag28252 |
Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
Calculated Molecular Weight | 102 kDa |
GenBank Accession Number | NM_010130 |
Gene Symbol | F4/80 |
Gene ID (NCBI) | 13733 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
Protocols
Product Specific Protocols | |
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IHC protocol for F4/80 antibody 81668-1-RR | Download protocol |
IF protocol for F4/80 antibody 81668-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
bioRxiv Integrin alpha1 beta1 promotes interstitial fibrosis in a mouse model of polycystic kidney disease | ||
Cell Rep The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring |