FcZero-rAb™ APC Anti-Mouse F4/80 Rabbit Recombinant Antibody

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  FC experiment of mouse peritoneal macrophages using APC-FcA98236

  1x10^6 mouse peritoneal macrophages were surface stained with 0.1 ug PE Anti-Mouse CD11b (M1/70) Rabbit IgG RecAb (PE-FcA65672, Clone: M1/70), and 0.1 ug APC Anti-Mouse F4/80 Rabbit RecAb (APC-FcA98236, Clone: 241959G4) or 0.1 ug APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using APC-FcA98236

  1x10^6 mouse peritoneal macrophages were surface stained with 0.1 ug APC Anti-Mouse F4/80 Rabbit RecAb (APC-FcA98236, Clone: 241959G4) (red) or 0.1 ug APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

F4/80 FcZero-rAb^TM Recombinant Antibody for FC

Cat No. APC-FcA98236

Clone No.241959G4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, Emr1

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98236 targets F4/80 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: F4/80 fusion protein Eg0788
• Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Calculated Molecular Weight: 102 kDa
• GenBank Accession Number: NM_010130.4
• Gene Symbol: F4/80
• Gene ID (NCBI): 13733
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.