Tested Applications
| Positive WB detected in | human plasma tissue, human blood tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66722-1-Ig targets Factor VIII in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15772 Product name: Recombinant human F8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2144-2351 aa of BC022513 Sequence: VFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY Predict reactive species |
| Full Name | coagulation factor VIII, procoagulant component |
| Calculated Molecular Weight | 2351 aa, 267 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC022513 |
| Gene Symbol | F8 |
| Gene ID (NCBI) | 2157 |
| RRID | AB_2882073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00451 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
F8, also named as FVIII, AHF, and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. FVIII is translated into a single-chain polypeptide (SCh) with the A1-A2-B-A3-C1-C2 domain, which can undergo glycosylation, sulfonation, and variable cleavage. The FVIII molecule is a heterodimer composed of a heavy chain (HCh, A1-A2-B domain, 90-210 kDa) and a light chain (LCh, A3-C1-C2 domain, 70-80 kDa)(PMID: 28109042; 18650448; 29292164). This antibody can recognize the light chain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Factor VIII antibody 66722-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Factor VIII Monoclonal antibody (1C11E11), catalog number 66722-1-Ig, has 4 total citations. These appear in Vet Res, J Thorac Dis, Front Oncol, and Res Pract Thromb Haemost. The associated research fields include coagulation cascade regulation in African swine fever virus, pulmonary microvascular endothelial cell transcriptomics in ARDS, mucinous gastric cancer organoid modeling, and immune tolerance in pediatric hemophilia A.
| Species | Application | Title |
|---|---|---|
Vet Res TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus
| ||
J Thorac Dis Transcriptome sequencing showed the differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome | ||
Front Oncol Establishment and characterization of patient-derived mucinous gastric cancer organoids with faithful histopathological features and reproducible drug response patterns. | ||
Res Pract Thromb Haemost Isolated A1-domain F8 large deletions are associated with favorable immune tolerance induction outcomes in children with severe hemophilia A. |
