FA2H Recombinant monoclonal antibody

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  WB analysis using 86020-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86020-2-RR (FA2H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86020-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86020-2-RR against Human FA2H were performed. The affinity constant is 0.127 nM.

FA2H Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86020-2-RR

Clone No.250401F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:1.14.18.-, FAAH, Fatty acid 2-hydroxylase, Fatty acid alpha-hydroxylase, Fatty acid hydroxylase domain-containing protein 1

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Tested Applications

• Positive WB detected in: MKN-45 cells, SGC-7901 cells, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86020-2-RR targets FA2H in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7717

  Product name: Recombinant human FA2H protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-164 aa of BC002679

  Sequence: MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHSANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEKYDEWVHQPVTRPIRLFHSDLIEGL

• Full Name: fatty acid 2-hydroxylase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC002679
• Gene Symbol: FA2H
• Gene ID (NCBI): 79152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7L5A8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).