FAAH Monoclonal antibody, PBS Only

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  WB analysis using 67607-1-Ig (same clone as 67607-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 67607-1-Ig (same clone as 67607-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IF Staining of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

FAAH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67607-1-PBS

Clone No.3C5B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C5B5, EC:3.1.1.-, EC:3.5.1.99, FAAH1, Fatty acid ester hydrolase

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Product Information

67607-1-PBS targets FAAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12720

  Product name: Recombinant human FAAH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 236-579 aa of BC093632

  Sequence: TDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

• Full Name: fatty acid amide hydrolase
• Calculated Molecular Weight: 579 aa, 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC093632
• Gene Symbol: FAAH
• Gene ID (NCBI): 2166
• RRID: AB_2882813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00519
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.