FAAH Monoclonal antibody

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  WB analysis using 67607-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of JAR using 67607-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67607-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of LNCaP using 67607-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FAAH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67607-1-Ig

Clone No.3C5B5

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C5B5, EC:3.1.1.-, EC:3.5.1.99, FAAH1, Fatty acid ester hydrolase

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Tested Applications

• Positive WB detected in: mouse brain tissue, JAR cells, LNCaP cells, rabbit brain tissue, pig liver tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67607-1-Ig targets FAAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12720

  Product name: Recombinant human FAAH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 236-579 aa of BC093632

  Sequence: TDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

• Full Name: fatty acid amide hydrolase
• Calculated Molecular Weight: 579 aa, 63 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC093632
• Gene Symbol: FAAH
• Gene ID (NCBI): 2166
• RRID: AB_2882813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O00519
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell Neurosci; Cannabinoid receptor 2 agonist AM1241 alleviates epileptic seizures and epilepsy-associated depression via inhibiting neuroinflammation in a pilocarpine-induced chronic epilepsy mouse model; Authors - Yiying Cai