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  • KD/KO Validated

FABP5 Monoclonal antibody

FABP5 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6E12

Cat no : 66299-1-Ig

Synonyms

E FABP, EFABP, FABP5, Fatty acid binding protein 5, PA FABP, PAFABP



Tested Applications

Positive WB detected inA375 cells, fetal human brain tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, mouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66299-1-Ig targets FABP5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FABP5 fusion protein Ag3005
Full Name fatty acid binding protein 5 (psoriasis-associated)
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC019385
Gene symbol FABP5
Gene ID (NCBI) 2171
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Protocols

Product Specific Protocols
WB protocol for FABP5 antibody 66299-1-IgDownload protocol
IHC protocol for FABP5 antibody 66299-1-IgDownload protocol
IF protocol for FABP5 antibody 66299-1-IgDownload protocol
FC protocol for FABP5 antibody 66299-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Med Mol Morphol

Expression of fatty-acid-binding protein 5 in intrahepatic and extrahepatic cholangiocarcinoma: the possibility of different energy metabolisms in anatomical location.

Authors - Risa Nakagawa
humanWB,IHC

Carcinogenesis

Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level.

Authors - Masafumi Ohira
  • KO Validated
humanWB

Front Oncol

Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.

Authors - Jinghui Lu