Validation Data Gallery
|Positive WB detected in||A375 cells, U2OS cells, rat brain tissue, fetal human brain tissue, A549 cells, HeLa cells, HEK-293 cells, mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue, mouse brown adipose tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66299-1-Ig targets FABP5 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||FABP5 fusion protein Ag3005|
|Full Name||fatty acid binding protein 5 (psoriasis-associated)|
|Calculated molecular weight||135 aa, 15 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC019385|
|Gene ID (NCBI)||2171|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)
|Product Specific Protocols|
|WB protocol for FABP5 antibody 66299-1-Ig||Download protocol|
|IHC protocol for FABP5 antibody 66299-1-Ig||Download protocol|
|IF protocol for FABP5 antibody 66299-1-Ig||Download protocol|
|FC protocol for FABP5 antibody 66299-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.
Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level.
Med Mol Morphol
Expression of fatty-acid-binding protein 5 in intrahepatic and extrahepatic cholangiocarcinoma: the possibility of different energy metabolisms in anatomical location.