FAF1 Monoclonal antibody

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• KD/KO Validated

FAF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2E1D7

Cat no : 60231-1-Ig

Synonyms

CGI 03, FAF1, FAS associated factor 1, hFAF1, HFAF1s, UBXD12, UBXN3A

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Validation Data Gallery

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  WB analysis of HEK-293 using 60231-1-Ig

  WB result of FAF1 antibody (60231-1-Ig, 1:5000) with si-Control and si-FAF1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 60231-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60231-1-Ig (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

60231-1-Ig targets FAF1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAF1 fusion protein Ag0407
• Full Name: Fas (TNFRSF6) associated factor 1
• Calculated Molecular Weight: 650aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC004970
• Gene Symbol: FAF1
• Gene ID (NCBI): 11124
• RRID: AB_11232212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.

Publications

Species	Application	Title
mouse	WB	J Am Heart Assoc; Endothelial Cell Cystathionine γ-Lyase Expression Level Modulates Exercise Capacity, Vascular Function, and Myocardial Ischemia Reperfusion Injury.; Authors - Huijing Xia
rat	WB,IF	Int Immunopharmacol; 6-Gingerol protects against cerebral ischemia/reperfusion injury by inhibiting NLRP3 inflammasome and apoptosis via TRPV1 / FAF1 complex dissociation-mediated autophagy.; Authors - Jing Luo