FAF2 Polyclonal antibody, PBS Only

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• FAF2 Polyclonal antibody, PBS Only (16251-1-PBS)

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  WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16251-1-AP (same clone as 16251-1-PBS)

  WB result of FAF2 antibody (16251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  IP result of anti-FAF2 (IP:16251-1-AP, 3ug; Detection:16251-1-AP 1:1000) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FAF2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16251-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

16251-1-PBS targets FAF2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9236

  Product name: Recombinant human FAF2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 97-445 aa of BC014001

  Sequence: YLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLPPEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSEEWPNPPTLQEAGLSHTEVLFVQDLTDE

• Full Name: Fas associated factor family member 2
• Calculated Molecular Weight: 445 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014001
• Gene Symbol: FAF2
• Gene ID (NCBI): 23197
• RRID: AB_2262469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CS3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.