FAM120C Polyclonal antibody

FAM120C Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25987-1-AP

Synonyms

CXorf17, FAM120C, ORF34, Protein FAM120C, Tumor antigen BJ HCC 21

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Validation Data Gallery

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  WB analysis of HepG2 using 25987-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25987-1-AP (FAM120C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 25987-1-AP

  IP result of anti-FAM120C (IP:25987-1-AP, 4ug; Detection:25987-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 25987-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25987-1-AP (FAM120C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25987-1-AP is FAM120C Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM120C fusion protein Ag23235
• Full Name: family with sequence similarity 120C
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC136413
• Gene Symbol: FAM120C
• Gene ID (NCBI): 54954
• RRID: AB_2880321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Neuron; Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes; Authors - Norah Al-Azzam