FAM127 Polyclonal antibody, PBS Only

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• FAM127 Polyclonal antibody, PBS Only (20282-1-PBS)

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  WB analysis of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20282-1-AP (same clone as 20282-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

  IP result of anti-FAM127 (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20282-1-AP (FAM127 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20282-1-AP (FAM127 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20282-1-AP (same clone as 20282-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

FAM127 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 20282-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

FAM127 A/B/C, CXX1, FAM127A, FAM127B, FAM127C

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Product Information

20282-1-PBS targets FAM127 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13930

  Product name: Recombinant human FAM127B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-113 aa of BC000393

  Sequence: MDGRVQLMKALLAGPLRPAARRWRNPIPFPETFDGDTDRLPEFIVQTCSYMFVDENTFSNDALKVTFLITRLTGPALQWVIPYIRKESPLLNDYRGFLAEMKRVFGWEEDEDF

• Full Name: family with sequence similarity 127, member B
• Calculated Molecular Weight: 113 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC000393
• Gene Symbol: FAM127B
• Gene ID (NCBI): 26071
• RRID: AB_10694677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWD3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.