FAM160B1 Polyclonal antibody

FAM160B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25388-1-AP

Synonyms

FHIP2A, FHF complex subunit HOOK interacting protein 2A

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Validation Data Gallery

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  WB analysis of PC-3 using 25388-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25388-1-AP (FAM160B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 25388-1-AP

  IP result of anti-FAM160B1 (IP:25388-1-AP, 5ug; Detection:25388-1-AP 1:300) with PC-3 cells lysate 1500ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25388-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25388-1-AP (FAM160B1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 25388-1-AP

  Immunofluorescent analysis of PC-3 cells using 25388-1-AP (FAM160B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25388-1-AP targets FAM160B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM160B1 fusion protein Ag22051
• Full Name: family with sequence similarity 160, member B1
• Calculated Molecular Weight: 765 aa, 86 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC037207
• Gene Symbol: FAM160B1
• Gene ID (NCBI): 57700
• RRID: AB_2880054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM160B1, also named as KIAA1600, is a 765 amino acid protein, which belongs to the UPF0518 family. The biological function of FAM160B1is still non-known. FAM160B1 exists as two isoforms and the molecular weight of isoform one is a 86 kDa protein.The isoforms two is produced by alternative splicing and is a 83 kDa protein. There are modified residues of FAM160B1 protein, so we detected 95 kDa and 100 kDa two bands by western blotting.