FAM160B2 Polyclonal antibody

FAM160B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17147-1-AP

Synonyms

Retinoic acid-induced protein 16, RAI16, FP13191, FHIP2B, FHF complex subunit HOOK-interacting protein 2B

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Validation Data Gallery

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  WB analysis of human brain using 17147-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17147-1-AP (FAM160B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17147-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17147-1-AP (FAM160B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17147-1-AP

  IP result of anti-FAM160B2 (IP:17147-1-AP, 4ug; Detection:17147-1-AP 1:300) with HepG2 cells lysate 3200ug.

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  IHC staining of human brain using 17147-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17147-1-AP (FAM160B2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17147-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17147-1-AP (FAM160B2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A549 using 17147-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17147-1-AP (FAM160B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17147-1-AP targets FAM160B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM160B2 fusion protein Ag10920
• Full Name: family with sequence similarity 160, member B2
• Calculated Molecular Weight: 743 aa, 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC012865
• Gene Symbol: FAM160B2
• Gene ID (NCBI): 64760
• RRID: AB_2246707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM160B2, also named as RAI16, may play a role in early lens development in mammals and in upregulation of genes in CD34+ cells in coronary artery disease patients.

Publications

Species	Application	Title
human	WB	Mol Cell Endocrinol; Identification of retinoid acid induced 16 as a novel androgen receptor target in prostate cancer cells.; Authors - Cui-Ling Ding