Piezo1 (extracellular domain) Recombinant antibody

Piezo1 (extracellular domain) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4B15

Cat no : 82625-4-RR

Synonyms

FAM38A, KIAA0233, Mib, PIEZO1, Piezo1 (extracellular domain)



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inrat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82625-4-RR targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Piezo1 (extracellular domain) fusion protein Ag7791
Full Name family with sequence similarity 38, member A
Calculated Molecular Weight 286 kDa
Observed Molecular Weight 233-286 kDa
GenBank Accession NumberBC008073
Gene Symbol Piezo1/FAM38A
Gene ID (NCBI) 9780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Protocols

Product Specific Protocols
WB protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
IHC protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
IF protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols