Piezo1 (extracellular domain) Recombinant antibody

Piezo1 (extracellular domain) Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4B15

Cat no : 82625-4-RR

Synonyms

FAM38A, KIAA0233, Mib, PIEZO1

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Validation Data Gallery

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  WB analysis using 82625-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Ag7791 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 82625-4-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Piezo1 (extracellular domain) antibody (82625-4-RR, Clone: 4B15 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IF detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82625-4-RR targets Piezo1 (extracellular domain) in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Piezo1 (extracellular domain) fusion protein Ag7791
• Full Name: family with sequence similarity 38, member A
• Calculated Molecular Weight: 286 kDa
• Observed Molecular Weight: 233-286 kDa
• GenBank Accession Number: BC008073
• Gene Symbol: FAM38A
• Gene ID (NCBI): 9780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)