FAM8A1 Polyclonal antibody

FAM8A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24746-1-AP

Synonyms

AHCP, FAM8A1, Protein FAM8A1

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Validation Data Gallery

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  WB analysis of HEK-293 using 24746-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24746-1-AP (FAM8A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24746-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24746-1-AP (FAM8A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 24746-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24746-1-AP (FAM8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24746-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24746-1-AP (FAM8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 24746-1-AP is FAM8A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM8A1 fusion protein Ag20476
• Full Name: family with sequence similarity 8, member A1
• Calculated Molecular Weight: 413 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC047881
• Gene Symbol: FAM8A1
• Gene ID (NCBI): 51439
• RRID: AB_2879702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
human	WB	Nat Commun; SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex; Authors - Liangguang Leo Lin