Tested Applications
| Positive WB detected in | HepG2 cells, Caco-2 cells, Raji cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22251-1-AP targets FAM98B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17747 Product name: Recombinant human FAM98B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-186 aa of BC093898 Sequence: LEEQALTKAAEGGLSSPEFSELCIWLGSQIKSLCNLEESITSAGRDDLESFQLEISGFLKEMACPYSVLISGDIKDRLKKKEDCLKLLLFLSTELQASQILQNKKHKNSQLDKNSEVYQEVQAMFDTLGIPKSTTSDIPHMLNQVESKVKDILSKVQ Predict reactive species |
| Full Name | family with sequence similarity 98, member B |
| Calculated Molecular Weight | 330 aa, 37 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC093898 |
| Gene Symbol | FAM98B |
| Gene ID (NCBI) | 283742 |
| RRID | AB_2879049 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q52LJ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAM98B (Family with Sequence Similarity 98 Member B) is a protein-coding gene that has been identified as a component of the human tRNA ligase complex (tRNA-LC), which plays a crucial role in tRNA splicing, RNA repair, and other cellular processes. FAM98B contains a glycine-rich intrinsically disordered region (IDR) that can aggregate, leading to the formation of pathological aggregates associated with neurodegenerative diseases. In a study, FAM98B was found to be depleted in patient tissues with abnormal tRNA splicing intermediates, indicating its role in these diseases. (PMID: 34854379, PMID: 40674500)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM98B antibody 22251-1-AP | Download protocol |
| IHC protocol for FAM98B antibody 22251-1-AP | Download protocol |
| IP protocol for FAM98B antibody 22251-1-AP | Download protocol |
| WB protocol for FAM98B antibody 22251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FAM98B Polyclonal antibody (Cat# 22251-1-AP) has 2 citations. They appear in Cell Discovery and Communications Biology. The cited research fields include RNA metabolism and tRNA splicing, as well as cuproptosis resistance involving lactylation and alternative polyadenylation. Both studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Discov NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance | ||
