Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 22251-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 22251-1-AP
IP result of anti-FAM98B (IP:22251-1-AP, 4ug; Detection:22251-1-AP 1:5000) with HepG2 cells lysate 1480 ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.
IF Staining of HepG2 using 22251-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FAM98B antibody (22251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FAM98B antibody (22251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
FAM98B (Family with Sequence Similarity 98 Member B) is a protein-coding gene that has been identified as a component of the human tRNA ligase complex (tRNA-LC), which plays a crucial role in tRNA splicing, RNA repair, and other cellular processes. FAM98B contains a glycine-rich intrinsically disordered region (IDR) that can aggregate, leading to the formation of pathological aggregates associated with neurodegenerative diseases. In a study, FAM98B was found to be depleted in patient tissues with abnormal tRNA splicing intermediates, indicating its role in these diseases. (PMID: 34854379, PMID: 40674500)
Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 22251-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 22251-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 22251-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.
IP Figures
IP experiment of HepG2 using 22251-1-AP
IP result of anti-FAM98B (IP:22251-1-AP, 4ug; Detection:22251-1-AP 1:5000) with HepG2 cells lysate 1480 ug.
IF/ICC Figures
IF Staining of HepG2 using 22251-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FAM98B antibody (22251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
FAM98B Polyclonal antibody
Catalog Number
22251-1-AP
Citations
2
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
