FARSA Monoclonal antibody

• 

  WB analysis using 67945-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67945-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67945-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67945-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67945-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67945-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 67945-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (67945-1-Ig, Clone: 2D3E2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

FARSA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67945-1-Ig

Clone No.2D3E2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FARSLA, FARSL, FARS, EC:6.1.1.20, CML33

---

Tested Applications

• Positive WB detected in: LNCaP cells, HSC-T6 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67945-1-Ig targets FARSA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12647

  Product name: Recombinant human FARSA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-300 aa of BC006495

  Sequence: MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTH

• Full Name: phenylalanyl-tRNA synthetase, alpha subunit
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006495
• Gene Symbol: FARSA
• Gene ID (NCBI): 2193
• RRID: AB_2918697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y285
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.