FARSA Monoclonal antibody, PBS Only

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  WB analysis using 67945-1-Ig (same clone as 67945-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67945-1-Ig (same clone as 67945-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67945-1-Ig (FARSA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67945-1-Ig (same clone as 67945-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67945-1-Ig (same clone as 67945-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67945-1-Ig (same clone as 67945-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67945-1-Ig (same clone as 67945-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67945-1-Ig (FARSA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67945-1-Ig (same clone as 67945-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FARSA antibody (67945-1-Ig, Clone: 2D3E2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67945-1-PBS in a different storage buffer formulation.

FARSA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67945-1-PBS

Clone No.2D3E2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FARSLA, FARSL, FARS, EC:6.1.1.20, CML33

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Product Information

67945-1-PBS targets FARSA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12647

  Product name: Recombinant human FARSA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-300 aa of BC006495

  Sequence: MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKGSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQIFLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVKRTH

• Full Name: phenylalanyl-tRNA synthetase, alpha subunit
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006495
• Gene Symbol: FARSA
• Gene ID (NCBI): 2193
• RRID: AB_2918697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y285
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.