Fibulin 1 Monoclonal antibody

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• Fibulin 1 Monoclonal antibody (68453-1-Ig)

Fibulin 1 Monoclonal Antibody for WB, ELISA

Cat No. 68453-1-Ig Copied!

Clone No. 1F4A1

Publications (1)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

FBLN1, 1F4A1, FBLN, FIBL 1, FIBL-1

• 

  WB analysis of human placenta using 68453-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68453-1-Ig (FBLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 68453-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68453-1-Ig (FBLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 68453-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 68453-1-Ig against Human FBLN1 was performed. The affinity constant is below 1 pM...

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human placenta tissue, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68453-1-Ig targets Fibulin 1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14249

  Product name: Recombinant human FBLN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC022497

  Sequence: MERAAPSRRVPLQLLLLGGLALLAAGVDADVLLEACCADGHRMATHQKDCSLPYATESKECRMVQEQCCHSQLEELHCATGISLANEQDRCATPHGDNASLEATFVKRCCHCCLLGRAAQAQGQSCEYSLMVGYQCGQVFRACCVKSQETGDLDVGGLQETDKIIEVEEEQEDPYLNDRCRGGGPCKQQCRDTGDEVVCSCFVGYQLLSDGVSCEDVNECITGSHSCRLGESCINTVGSFRCQRDSSCGTGYELTEDNSCKDIDECESGIHNCLPDFICQNTLGSFRCRPKLQCKSGFIQ

• Full Name: fibulin 1
• Calculated Molecular Weight: 703 aa, 77 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC022497
• Gene Symbol: FBLN1
• Gene ID (NCBI): 2192
• RRID: AB_3085165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P23142
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Fibulin 1 Monoclonal antibody (1F4A1), catalog number 68453-1-Ig, has 1 total citation. The cited publication appeared in Frontiers in Bioengineering and Biotechnology (2025), investigating how excessive DAO inhibits myoblast migration, impairs myotube fusion, and contributes to muscle strength decline by reducing extracellular matrix components. The research field is muscle biology and bioengineering, with the antibody used in Western blot for mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Front Bioeng Biotechnol; Excessive DAO inhibits myoblast migration, leading to impaired myotube fusion and muscle strength decline by reducing ECM.; Authors - Xiang Liu