FBP1 Polyclonal antibody, PBS Only

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• FBP1 Polyclonal antibody, PBS Only (12842-1-PBS)

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  WB analysis of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

  WB result of FBP1 antibody (12842-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBP1 transfected MCF-7 cells. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  WB analysis using 12842-1-AP (same clone as 12842-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12842-1-AP (same clone as 12842-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12842-1-AP (FBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 12842-1-AP (same clone as 12842-1-PBS)

  IP result of anti-FBP1 (IP:12842-1-AP, 4ug; Detection:12842-1-AP 1:10000) with mouse liver tissue lysate 2480 ug. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 12842-1-AP (same clone as 12842-1-PBS)

  IP result of anti-FBP1 (IP:12842-1-AP, 4ug; Detection:12842-1-AP 1:10000) with MCF-7 cells lysate 1200 ug. This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12842-1-AP (same clone as 12842-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12842-1-AP (FBP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12842-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FBP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12842-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, EC:3.1.3.11, FBP, FBPase 1, Fructose-1,6-bisphosphatase 1

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Product Information

12842-1-PBS targets FBP1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3837

  Product name: Recombinant human FBP1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-338 aa of BC012927

  Sequence: MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

• Full Name: fructose-1,6-bisphosphatase 1
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC012927
• Gene Symbol: FBP1
• Gene ID (NCBI): 2203
• RRID: AB_2103572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09467
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).