FBX15 Polyclonal antibody, PBS Only

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• FBX15 Polyclonal antibody, PBS Only (13024-1-PBS)

• 

  WB analysis of A2780 using 13024-1-AP (same clone as 13024-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 13024-1-AP (same clone as 13024-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13024-1-AP (FBX15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13024-1-AP (same clone as 13024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13024-1-AP (FBX15 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13024-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FBX15 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 13024-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

FBXO15, F-box only protein 15, F box protein 15, F box only protein 15

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Product Information

13024-1-PBS targets FBX15 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3564

  Product name: Recombinant human FBXO15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC029579

  Sequence: MPSEILLKIFSYLDAVSLLCTGCVSRRFYHLANDNFIWIGIYSTAFSPARSNWKFNSVEKIAMSMSFLSVQDKEAGYWKKEYITKQIASVKAALADILKPVNPYTGLPVKTKEALRIFGLGWAIILKEKGGKEYIMEHVDLSINDTSVTVIWYGKKWPCLASLSTLDLCGMTPVFTDWYKTPTKHRLRWHSLIAKYNLSHLTISTMIGCDRLIRIFCLHPGLLVGVWKKEEELAFVMANLHFHHLVERSTLGSATIPYELPPHSPFLDDSPEYGLHGYQLHVDLHSGGVFYLCGTFRNLFT

• Full Name: F-box protein 15
• Calculated Molecular Weight: 434 aa, 49 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC029579
• Gene Symbol: FBX15
• Gene ID (NCBI): 201456
• RRID: AB_2104120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCQ5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX15 (or FBX15) belongs to the FBXs class, and 2 isoforms produced by alternative splicing has been described. This antibody is expected detect both isoforms.