Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
24282-1-AP targets FBXO28 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14900 Product name: Recombinant human FBXO28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 69-368 aa of BC031576 Sequence: IVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRKSKRLRNRK Predict reactive species |
| Full Name | F-box protein 28 |
| Calculated Molecular Weight | 368 aa, 41 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC031576 |
| Gene Symbol | FBXO28 |
| Gene ID (NCBI) | 23219 |
| RRID | AB_2879469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FBXO28 also named as F box only protein 28 is a 368 amino-acid protein, which contain 1 F-box domain and belongs to F-box protein family. FBXO28 is a critical component of SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. FBOX28 encodes a cell cycle regulated protein with a critical function in tumor cell proliferation. FBXO28 assembles an SCF-FBXO28 ubiquitin ligase whose activity is required during progression through the cell cycle by cyclin-dependent kinase 1/2 mediated phosphorylation. FBXO28 expression and phosphorylation to be strongly associated with poor prognosis and worse overall survival in human breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FBXO28 antibody 24282-1-AP | Download protocol |
| WB protocol for FBXO28 antibody 24282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
