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FBXW11 Polyclonal antibody, PBS Only (13149-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 13149-1-AP (same clone as 13149-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
WB analysis using 13149-1-AP (same clone as 13149-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
WB analysis of SGC-7901 using 13149-1-AP (same clone as 13149-1-PBS)
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
WB analysis using 13149-1-AP (same clone as 13149-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
WB analysis of SGC-7901 using 13149-1-AP (same clone as 13149-1-PBS)
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
WB analysis of human stomach using 13149-1-AP (same clone as 13149-1-PBS)
human stomach tissue were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
WB analysis using 13149-1-AP (same clone as 13149-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13149-1-AP (FBXW11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 13149-1-AP (same clone as 13149-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 13149-1-AP (same clone as 13149-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13149-1-AP (FBXW11 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
IF Staining of HCT 116 using 13149-1-AP (same clone as 13149-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 13149-1-AP (same clone as 13149-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 13149-1-AP (same clone as 13149-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXW11 antibody (13149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13149-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

FBXW11 Polyclonal antibody, PBS Only

Name: FBXW11 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13149-1-PBS

FBXW11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13149-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

13149-1-PBS targets FBXW11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3761 Product name: Recombinant human FBXW11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-529 aa of BC026213 Sequence: NLQRIQCRSENSKGVYCLQYDDEKIISGLRDNSIKIWDKTSLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPSAQNETRSPSRTYTYISR Predict reactive species
Full Name	F-box and WD repeat domain containing 11
Calculated Molecular Weight	542 aa, 61 kDa
Observed Molecular Weight	61 kDa
GenBank Accession Number	BC026213
Gene Symbol	FBXW11
Gene ID (NCBI)	23291
RRID	AB_2102595
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UKB1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.