FCHSD1 Polyclonal antibody

FCHSD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23362-1-AP

Synonyms

FCH and double SH3 domains 1, FCHSD1, NWK2

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Validation Data Gallery

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  WB analysis using 23362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23362-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23362-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23362-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 23362-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using FCHSD1 antibody (23362-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23362-1-AP targets FCHSD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FCHSD1 fusion protein Ag19963
• Full Name: FCH and double SH3 domains 1
• Calculated Molecular Weight: 690 aa, 77 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC047016
• Gene Symbol: FCHSD1
• Gene ID (NCBI): 89848
• RRID: AB_2879265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FCHSD1 (FCH and double SH3 domains 1), also known as NWK2, is a member of the F-BAR family, which is involved in endocytosis and vesicle trafficking. FCHSD1 is expressed in mouse cochlear sensory hair cells and plays an important role in regulating the assembly or maintenance of F-actin in hair cell stereocilia and cuticular plate (PMID: 23437151). FCHSD1 also plays a role in stress response sensing and oxidative stress integration and is involved in the pathogenesis of emphysema (PMID: 34168078). FCHSD1 has three isoforms with molecular weights of 77 kDa, 46 kDa, and 40 kDa.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki