Ficolin-3 Polyclonal antibody

Ficolin-3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11867-1-AP

Synonyms

Hakata antigen, HAKA1, FCNH, FCN3, Collagen/fibrinogen domain-containing protein 3

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Validation Data Gallery

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  WB analysis of human plasma using 11867-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 11867-1-AP (Ficolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 11867-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ficolin-3 antibody (11867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: human plasma
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

11867-1-AP targets Ficolin-3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ficolin-3 fusion protein Ag2449
• Full Name: ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC020731
• Gene Symbol: Ficolin-3
• Gene ID (NCBI): 8547
• RRID: AB_2104432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains (PMID: 20375620). They are innate pattern recognition receptors and play integral roles within the innate immune response. In humans, there are three ficolins termed M-, L-, and H-ficolin (also referred to as ficolin-1, -2, and -3) whereas rodents only possess ficolin-A and -B, which are the orthologues of human L- and M-ficolin, respectively (PMID: 30868077). Ficolin-3 (Hakata antigen or H-ficolin) was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus (PMID: 1859827). It has been shown to have a calcium-independent lectin activity. Ficolin-3 is associated with MASPs and sMAP, and which activates the lectin pathway (PMID: 11907111). Ficolin-3 shows affinity for GalNAc, GlcNAc, D-fucose and galactose (PMID: 30868077).

Publications

Species	Application	Title
human	WB	Nat Commun; Secretome profiling reveals acute changes in oxidative stress, brain homeostasis, and coagulation following short-duration spaceflight; Authors - Nadia Houerbi
human,rat	WB	Front Mol Biosci; Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.; Authors - Huafeng Song
human	IHC	Int J Med Sci; Loss of ficolin-3 expression is associated with poor prognosis in patients with hepatocellular carcinoma; Authors - Chia-Chi Chen
mouse,human	WB,IHC	J Exp Clin Cancer Res; Ficolin 3 promotes ferroptosis in HCC by downregulating IR/SREBP axis-mediated MUFA synthesis; Authors - Yanmei Yuan