FECH Polyclonal antibody

FECH Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14466-1-AP


EPP, FCE, FECH, Ferrochelatase, mitochondrial, Heme synthase, Protoheme ferro lyase

Tested Applications

Positive WB detected inK-562 cells, human liver tissue, HepG2 cells, human heart tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 12 publications below

Product Information

14466-1-AP targets FECH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FECH fusion protein Ag5814
Full Name ferrochelatase (protoporphyria)
Calculated molecular weight 48 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC039841
Gene symbol FECH
Gene ID (NCBI) 2235
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.


Product Specific Protocols
WB protocol for FECH antibody 14466-1-APDownload protocol
IHC protocol for FECH antibody 14466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio

Brain Commun

Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Authors - Nunziata Maio

Cell Death Dis

Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells.

Authors - Jun Jing

Nat Metab

Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells.

Authors - Andrew J Schwartz

Cell Rep

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Authors - Michelle Grace Acoba


Involvement of protoporphyrin IX accumulation in the pathogenesis of isoniazid/rifampicin-induced liver injury: the prevention of curcumin.

Authors - Leiyan He