FECH Polyclonal antibody

FECH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 14466-1-AP


EPP, FCE, FECH, Ferrochelatase, mitochondrial, Heme synthase, Protoheme ferro lyase

Tested Applications

Positive WB detected inmouse heart tissue, human liver tissue, HepG2 cells, human heart tissue, K-562 cells, mouse kidney tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14466-1-AP targets FECH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FECH fusion protein Ag5814
Full Name ferrochelatase (protoporphyria)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 40-48 kDa
GenBank Accession NumberBC039841
Gene Symbol FECH
Gene ID (NCBI) 2235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.


Product Specific Protocols
WB protocol for FECH antibody 14466-1-APDownload protocol
IHC protocol for FECH antibody 14466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang

Cancer Discov

NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins

Authors - Naiara Santana-Codina

Cell Metab

A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.

Authors - Nunziata Maio

Mol Cell

Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.

Authors - Ross A Weber

J Am Coll Cardiol

Heme levels are increased in human failing hearts.

Authors - Khechaduri Arineh A

Nat Commun

PMID: 32019928

Authors - Erika M Palmieri